If you need assistance with selecting the best format contact our expert technical support team. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. This data display is provided for general comparisons between formats. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.Ĩ999 BioLegend Way, San Diego, CA 92121 Toll-Free Phone: 1-877-Bio-Legend ( 246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587 By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. *These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. Please contact Technical Service if you have any questions. The dye molecule remains the same, so you should expect the same quality and performance from our Cyanine3 products. This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.īioLegend is in the process of converting the name C圓 to Cyanine3. It is recommended that the reagent be titrated for optimal performance for each application. The antibody solution should be stored undiluted between 2☌ and 8☌, and protected from prolonged exposure to light. The antibody was purified by affinity chromatography, and conjugated with Cyanine3 under optimal conditions. Asp f1 reactivity may relate to the presence of actively growing Aspergillus spp., which might be a useful marker for guiding antifungal therapy in ABPA.ĪBPA Aspergillus bronchitis cystic fibrosis recombinant antigens serology.Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA). The use of multiple recombinant antigens may improve the diagnostic accuracy in CF complicated with ABPA or AB. Aspf1 IgG reactivity in ABPA patients correlated with positive culture. There were very low IgE antibody levels against all recombinant antigens in patients with AS. A hyperimmunization reaction may also cause this type of result. If all three immunoglobulins have high results, then this can be because of an infection, an autoimmune disorder, or inflammatory disorders. Patients with AB expressed higher IgG positivity to Asp f1 and Asp f2 compared with those with ABPA. When an increase in just IgG is seen, then this is typically due to leukemia, multiple myeloma, or lymphoma. The ABPA group showed significantly greater IgE reactivity to Asp f1, f2, f3 and f4 compared to patients with AS. Antibodiesincluding IgM, IgG, and IgAagainst S and its subunits can be detected in serum within 1-3 weeks after infection (7, 8). Serum IgE and IgG antibodies were measured in 12 CF patients with allergic bronchopulmonary aspergillosis (ABPA), 12 with Aspergillus sensitization (AS) and 12 with Aspergillus bronchitis (AB) against recombinant antigens Asp f1, f2, f3, f4 and f6. RBD is the main target for neutralizing antibodies. fumigatus recombinant antigens in CF patients with different Aspergillus diseases. In this study, we dissected the IgE and IgG responses to multiple A. This is further complicated by inconsistent antibody reactivity to the currently used crude antigen, which has led a more focused evaluation of the efficacy of IgE response to a number of pure Aspergillus fumigatus recombinant proteins in patients with CF and asthma. The diagnosis of aspergillosis in cystic fibrosis (CF) patients remains a challenge due to overlapping features of both diseases.
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